Screening and Preliminary Phytochemicals Study of Microlepia speluncae (L.) Moore collected from Ponmudi, Kerala, India

Amster Regin Lawrence R 1, John Peter Paul J 2,*

1Research Scholar, Centre for Advanced Research in Plant Sciences (CARPS), Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.
2Assistant Professor of Botany and Director, Centre for Advanced Research in Plant Sciences (CARPS), St. Xavier’s College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.

Abstract:The present study was intended to discover the preliminary phytochemicals of Microlepia speluncae (L.) Moore from Ponmudi, India. The preliminary phytochemical analysis was conducted in five extracts namely ethanol, acetone, ethyl acetate, chloroform and hexane by Harborne method. The preliminary phytochemical analysis showed the presence of alkaloids, anthocyanin, anthroquinone, catechin, cardiac glycosides, coumarins, flavonoids, glycosides, phenolic groups, phlobatannins, phytosteroids, quinones, saponins, tannins, terpenoids, emodins and diterpenes. Among the various phytochemicals studied, flavonoids, terpenoids and diterpenes showed the maximum presence, being found in five different extracts and catechin was observed in only one extract. From the results, it was noted that the extracts of Microlepia speluncae (L.) Moore was found to be the presence of a number of active secondary metabolites. This report will lead to the isolation and characterization of these active secondary metabolites for bio-efficacy and bioactivity.

Keywords: Phytochemical, Bioactive compounds, Microlepia speluncae, Pteridophytes, Dennstaedtiaceae


  1. Manickam VS, Irudayaraj V. Pteridophyte flora of Western Ghats of South India. BI publications, New Delhi. 1992; Pp: 652.
  2. De-Fatima A, Modolo LV, Conegero LS, Pili RA, Ferreira CV, Kohn LK. Lactones and their derivatives, biological activities, mechanisms of action and potential leads for drug design. Curr Med Chem 2006; 13:3371-84.
  3. Zengu, LI, Huil H, Hang Z, Jun Z. Review on the extraction of flavonoids from fern. J Sanm Univ 2008; 25:22.
  4. Pratibha P, Sophia D, Perumal PC, Gopalakrishnan VK. In silico docking analysis of Emilia sanchifolia (L.) DC. Gas chromatography-mass spectrometry derived terpenoids compounds against pancreatic cancer targets (AKT and BRCA2). World J Pharm Sci 2014; 3:1844-55.
  5. Chen JJ, Duh CY, Chen JF. New cytotoxic biflavonoids from Selaginella delicatula. Planta Medica 2005; 71(7):659-65.
  6. Molina M, Garcia VR, Santayana MP. Local knowledge and management of the royal fern (Osmunda regalis L.) in Northern Spain: implications for biodiversity conservation. Am Fern J 2009; 99:45-55.
  7. Harborne JB. Phytochemical methods: A guide to modern techniques of plants analysis, Chapman & Hall. London, Ltd. 1998; 1-188.
  8. John Peter Paul J, Yuvaraj P. Phytochemical analysis of Padina distromatica Hauck. Indo Am J Pharm Res 2013; 3:5290-7.