All in one Thermoascus aurantiacus and its Industrial Applications

Marwa O Elnahas, Waill A Elkhateeb*, Ghoson M Daba

Chemistry of Natural and Microbial Products Department, Pharmaceutical Industries Division, National Research Centre, Dokki, Giza, 12622, Egypt.

Abstract: Background: Fungi are well known biotechnological tools that have various applications in the fields of industry. Thanks to their ability to produce set of prestigious enzymes that is eco-friendly and can replace harmful chemicals used in those industries. Thermoascus is an ascomycetous fungus that belongs to family Trichocomaceae, which is famous for its promising mycotechnological applications due to its capability to produce potent heat stable enzymes such as cellulases, xylanases, and β-glucosidases. Object: The aim of this review is to highlight the description. ecology, and important industrial applications of the genus Thermoascus in general, and the species Thermoascus aurantiacus in particular focusing on its heat-resistant hydrolase enzymes that have different potential biotechnological applications. Conclusion: Thermoascus originated metabolites are of potential biological activities especially as antioxidant agents. Furthermore, enzymes produced by Thermoascus are involved as promising tool in many important mycotechnological applications such as food, textile, paper, pulp, animal feed, conversion of biomass into biofuels, as well as other chemical industries. Understanding the importance of these fungus thermostable enzymes may contribute in encouraging for further studies in order to employ them in new biotechnological fields. Enhancing the production of industrial thermostable enzymes from Thermoascus aurantiacus via the application of different statistical approaches and newly developed molecular biology methods is of critical importance. Furthermore, more effort should be directed towards introducing Thermoascus aurantiacus into new hosts for further studying the deconstruction of plant cell wall.

Keywords:Thermoascus aurantiacus, Thermostable enzymes, Industrial application, Biofuel.


  1. Apinis A. Dactylomyces and Thermoascus. Transactions of the British Mycological Society, 1967; 50:573-82.
  2. Béguin P. Aubert J-P. The biological degradation of cellulose. FEMS microbiology reviews 1994; 13:25-58.
  3. Berka RM, Grigoriev IV, Otillar R, Salamov A, Grimwood J, Reid I, Ishmael N, John T, Darmond C, Moisan M-C. Comparative genomic analysis of the thermophilic biomass-degrading fungi Myceliophthora thermophila and Thielavia terrestris. Nat Biotech 2011; 29: 922–7.
  4. Bertleff W, Neumann P, Baur R, Kiessling D. Aspects of polymer use in detergents. Journal of Surfactants and Detergents 1998; 1:419-24.
  5. Bhat M, Bhat S. Cellulose degrading enzymes and their potential industrial applications. Biotechnol Advan. 1997: 15: 583-620.
  6. Bhat MK, Parry NJ, Kalogiannis S, Beever DE, Owen E. Thermostable Cellulase and Xylanase from Thermoascus aurantiacus and Their Potential Industrial Applications. In Glycosyl Hydrolases for Biomass Conversion vol. 769. Washington D C: American Chemical Society. 2000:204–21.
  7. Brienzo M, Arantes V, Milagres AM. Enzymology of the thermophilic ascomycetous fungus Thermoascus aurantiacus. Fung Biol Rev 2008; 22:120–30.
  8. Bringer S, Sprey S, Sahm H. Purification and properties of alcohol oxidase from Poria contigua. Euro J Biochem. 1979. 101:563-70.
  9. Couderc R. Baratti J. Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the alcohol oxidase. Agri Biolog chem. 1980; 44:2279-89.
  10. Da Silva R, Lago EL, Merheb CW, Macchione MM, Park YK, Gomes E. Production of xylanase and CMCase on solid state fermentation in different residues by Thermoascus aurantiacus. Brazilian J Microbiol 2005; 36: 235–41.
  11. Deploey JJ, 1995. Some factors affecting the germination of Thermoascus aurantiacus ascospores. Mycol 87: 362–5.
  12. Elkhateeb WA, Zohri AA, Mazen MB, Hashem M, Daba GM. Investigation of diversity of endophytic, phylloplane and phyllosphere mycobiota isolated from different cultivated plants in new reclaimed soil, Upper Egypt with potential biological applications. J Med Pharm Res 2016; 2:23-31.‏
  13. Elkhateeb WA. Some mycological, phytopathological and physiological studies on mycobiota of selected newly reclaimed soils in Assiut Governorate, Egypt (M. Sc. Thesis, Faculty of Science, Assuit University, Egypt. 2005; p 238.
  14. Himmel ME, Ruth MF, Wyman CE. 1999. Cellulase for commodity products from cellulosic biomass. Curr Opinion in Biotechnol 10: 358–63.
  15. Ichikawa Y, Look GC, Wong C-H. Anal. Biochem. 1992; 202:215-38.
  16. Jain KK, Dey TB, Kumar S, Kuhad RC. Production of thermostable hydrolases (cellulases and xylanase) from Thermoascus aurantiacus RCKK: a potential fungus. Bioproc Biosys Engin 2015; 38:787-96.‏
  17. Kalogeris E, Christakopoulos P, Katapodis P, Alexiou A, Vlachou S, Kekos D, Macris BJ. Production and characterization of cellulolytic enzymes from the thermophilic fungus Thermoascus aurantiacus under solid state cultivation of agricultural wastes. Proc Biochem 2003; 38:1099-104.‏
  18. Ko HS, Fujiwara H, Yokoyama Y, Ohno N, Amachi S, Shinoyama H et al. Inducible production of alcohol oxidase and catalase in a pectin medium by Thermoascus aurantiacus IFO 31693. J Biosci Bioeng 2005; 99:290-2.‏
  19. Kocabas A, Ogel Z,Bakir U. Xylanase and itaconic acid production by Aspergillus terreus NRRL 1960 within a biorefinery concept. Ann of microbiol 2014; 64:75-84.
  20. Kuhad RC, Singh A, Eriksson K-EL. Microorganisms and enzymes involved in the degradation of plant fiber cell walls, in Biotechnology in the pulp and paper industry. Springer (Vol 1997; pp. 45-125).
  21. Maheshwari R, Bharadwaj G, Bhat M. Thermophilic fungi: their physiology and enzymes. Microbiol Molec Biol Rev. 2000; 64:461-88.
  22. McClendon SD, Batth T, Petzold CJ, Adams PD, Simmons BA, Singer SW. Thermoascus aurantiacus is a promising source of enzymes for biomass deconstruction under thermophilic conditions. Biotechnol Biofuel 2010; 54: 1-9.
  23. Miehe H. 1907. Die selbsterhitzung des heus: Eine biologische studie. G. Fischer; 1907.
  24. Milagres AM, Santos E, Piovan T, Roberto IC. Production of xylanase by Thermoascusaurantiacus from sugar cane bagasse in an aerated growth fermentor. Process Biochem 2004; 39:1387–91.
  25. Monte JR, Carvalho W, Milagres AM. Use of a mixture of thermophilic enzymes produced by the fungus Thermoascus aurantiacus to enhance the enzymatic hydrolysis of the sugarcane bagasse cellulose. Am J Agric Biol Sci 2010; 5:468-76.‏
  26. Nakagawa T, Mizumura T, Mukaiyama H, Miyaji T, Yurimoto H, Kato N, Tomizuka N. Physiological role of the second alcohol oxidase gene MOD2 in the methylotrophic growth of Pichia methanolica. Yeast, 2002; 19:1067-73.‏
  27. Nakagawa T, Uchimura T, Komagata K. Isozymes of methanol oxidase in a methanol-utilizing yeast, Pichia methanolica IAM 12901. J Fermn Bioeng 1996; 81:498-503.
  28. Olofsson K, Wiman M, Lidén G. Controlled feeding of cellulases improves conversion of xylose in simultaneous saccharifications and co-fermentation for bioethanol production. J Biotechnol 2010; 145:168-75.
  29. Sannia G, Limongi P, Cocca E, Buonocore F, Nitti G, Giardina P. Purification and characterization of a veratryl alcohol oxidase enzyme from the lignin degrading basidiomycete Pleurotus ostreatus. Biochimica et Biophysica Acta (BBA)-General Subjects, 1991; 1073:114-9.‏
  30. Santos E, Piovan T, Roberto IC, Milagres AM. Kinetics of the solid state fermentation of sugarcane bagasse by Thermoascus aurantiacus for the production of xylanase. Biotechnol Letters 2003; 25:13–6.
  31. Schuerg T, Gabriel R, Baecker N, Baker S, Singer S. Thermoascus aurantiacus is an Intriguing Host for the Industrial Production of Cellulases. Curr Biotechnol 2017; 6:89-97.
  32. Schuerg T, Prahl JP, Gabriel R, Harth S, Tachea F, Chen CS, Mirshiaghi M. Xylose induces cellulase production in Thermoascus aurantiacus. Biotechnol for biofuels 2017; 10:1-11.‏
  33. Sharma HS. Economic importance of thermophilous fungi. Appl Microbiol Biotechnol. 1989; 31:1-10.
  34. Shinoyama H, Takei k, Andō A, Fujii T, Sasaki M, Doi M, Yasui T. Enzymatic synthesis of useful alkyl β-glucosides. Agricul biological chem 1991; 55:1679-81.
  35. Silva RD, Lago ES, Merheb CW, Macchione MM, Park YK, Gomes E. Production of xylanase and CMCase on solid state fermentation in different residues by Thermoascus aurantiacus miehe. Brazilian J Microbiol 2005; 36:235-41.‏
  36. Sugden C, Bhat M. Cereal straw and pure cellulose as carbon sources for growth and production of plant cell-wall degrading enzymes by Sporotrichum thermophile. W J Microbiol Biotechnol 1994; 10:444-51.
  37. Suye S-I. Purification and properties of alcohol oxidase from Candida methanosorbosa M-2003. Curr microbiol. 1997; 34: 374-7.
  38. Szijártó N, Siika-Aho M, Tenkanen M, Alapuranen M, Vehmaanperä J, Réczey K, Viikari L. Hydrolysis of amorphous and crystalline cellulose by heterologously produced cellulases of Melanocarpus albomyces. J biotechnol 2008; 136:140-7.
  39. Tani Y, Vongsuvanlert V. Kumnuanta J. Raw cassava starch-digestive glucoamylase of Aspergillus sp. N-2 isolated from cassava chips. J Ferment Technol 1986; 64:405-10.
  40. Upadhyay J, Farmelo M, Goetz S, Melan M. A new variety of a thermophilic mold, Thermoascus aurantiacus var. levisporus. Mycopathol 1984; 87:71-80.
  41. Viikari L, Alapuranen M, Puranen T, Vehmaanperä J, Siika-aho M. Biofuels: Advances in Biochemical Engineering/Biotechnology. In Thermostable Enzymes in Lignocellulose Hydrolysis Biofuels. vol. 108th edition. Edited by Olsson L. Berlin/Heidelberg: Springer; 2007:121–45.
  42. Vonck J, van Bruggen E. Electron microscopy and image analysis of two-dimensional crystals and single molecules of alcohol oxidase from Hansenula polymorpha. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1990; 1038:74-9.
  43. Waters DM, Murray PG, Ryan LA, Arendt EK, Tuohy MG. Talaromyces emersonii Thermostable Enzyme Systems and Their Applications in Wheat Baking Systems. J Agricul Food Chem 2010; 58:7415–22.
  44. Yu J-H, Keller N. Regulation of secondary metabolism in filamentous fungi. Annu Rev Phytopathol 2005; 43:437-58.
  45. Zeikus J. In Enzymes in Biomass Conversion, Leatham, GF; Himmel, ME Eds. in ACS Symp. Amercian Chem. Soc., Washington, DC. 1991; (Vol. 460, pp. 36-52).‏