Antidiabetic and Nephroprotective Activity of Hedychium spicatum in Streptozotocin-Induced Diabetic Rats

Roopal Mittal1,2,*, Ankita Beniwal3

1R.K.S.D. College of Pharmacy, Ambala Road, Kaithal (Haryana) 136027, India.
2Research Scholar, IKG Punjab Technical University, Jalandhar (Punjab) 144601,India.
3Patient Safety Pharmacovigilance Associate, ADR-Monitoring Centre-KCGMC Karnal (Haryana) 132001, India.

Abstract: Objective: Diabetic Nephropathy, an end-stage renal disease clinically manifested as progressively increased proteinuria accompanied by increased blood pressure. Histologically, Diabetic Nephropathy is characterised by diffuse or nodular glomerulosclerosis, tubule-interstitial fibrosis and atrophy. The aim of this study was to identify either the ethanolic extract posses an antidiabetic activity and ameliorate effect on diabetes-induced nephropathy or not. Material and method: In-vitro alpha-amylase inhibitory assay and oral glucose tolerance tests were carried out before the induction of diabetes. Fasting blood glucose was measured at different time intervals to check the hyperglycaemic state. On the 40th day of the study, the urine sample was collected from metabolic cages and venous blood samples were obtained and used for determination of various biochemical parameters. In the end, histopathological studies were carried for checking the preventative effect on kidneys. Result: The In-Vitro α-Amylase inhibitory assay results revealed that the ethanolic extracts were found to have significant inhibitory potential as compared to acarbose. Further the OGTT, Serum BUN, creatine, lipid peroxidation, lipid profile except High Density Lipoprotein-C and anti-oxidant were found to be decreased in a dose-dependent manner, marked effect in Group VII (50% extract of 100 mg/kg and 50% of standard dose). In histopathology, Group VI and VII prevented degeneration of nephrons in the kidney and maintained the normal cellular size of nephrons. Conclusion: The ethanolic extract posses a significant activity to lower the blood glucose level as well protect the renal damage.

Keywords:Diabetic nephropathy, anti-oxidant, lipid peroxidation, Oral Glucose Tolerance Test, Histopathology.


  1. Tierney LM, McPhee SJ, Papadakis MA. Current Medical Diagnosis & Treatment. International Edition. Lange Medical Books/McGraw-Hill, New York; 2002: 1203-15.
  2. Anonymous. Diabetes now a global threat gets own day. Sunday Times of India 2006; 24:11.
  3. Schalkwijk GC, Stehouwer DC. Vascular complications in diabetes mellitus: the role of endothelial dysfunction. Clin Sci 2005; 109:143-59.
  4. Alsaad KO, and Herzenberg AM. Distinguishing Diabetic nephropathy from other causes of glomerulosclerosis: an update. J Clin Pathol 2007; 60: 18-26.
  5. Marles RJ, Farnsworth NR. Antidiabetic plants and their active constituents. Phytomedicine 1995; 2:137-89.
  6. Chopra RN, Nayar SL, Chopra LC. Glossary of Indian Medicinal Plants. Council of Scientific & Industrial Research, 1986; New Delhi.
  7. Srimal RC, Sharma SC, Tandon JS. Anti-inflammatory and other pharmacological effects of Hedychium spicatum (Buch-Hem). Indian J Pharmacol 1984; 16: 143–7.
  8. Dhar ML, Dhar MM, Dhawan BN, Mehrotra BN, Srimal RC, Tandon JS. Screening of Indian medicinal plants for biological activity: Part IV. Indian J Exp Biol 1973; 11: 43-54.
  9. Giri D, Tamta S, Pandey A. A review account on medicinal value of Hedychium spicatum buch-ham ex sm vulnerable medicinal plant. J Med Plants Res 2010; 2773-77.
  10. Reddy PP, Rao RR, Shashidhar J, Sastry BS, Rao JM, Babu KS. Phytochemical investigation of labdane diterpenes from the rhizomes of Hedychium spicatum and their cytotoxic activity. Bioorg Med Chem Lett 2009; 19: 6078-81.
  11. Khandelwal KR. Practical Pharmacognosy techniques and experiments. Pune: Nirali Prakashan; 2008; 8: 149‑56.
  12. Kokate CK. Practical Pharmacognosy. New Delhi: Vallabh Prakashan; 2005, 107-13.
  13. Raman N. Phytochemical techniques. New India Publishing agency, Pitampura, New Delhi, India, 2006, 19-24.
  14. Giancarlo S, Rosa LM, Nadjafi F, Francesco M. Hypoglycaemic activity of two spices extracts: Rhus coriaria L. and Bunium Persicum Boiss. Nat Prod Res 2006; 882-6.
  15. Quershi S, Shah AH, Aeegel AM. Toxicity studies on Alpinia galanga and Curcuma longa. Planata med 1992; 58: 124-7.
  16. Du Vigneaud V, Karr WG. Carbohydrate utilization, rat of disappearance of D-glucose from the blood. J Bio Chem 1925; 66: 281-300.
  17. Burstein M, Scholnick HR, Morfin R. Rapid method for the isolation of lipoproteins from serum by precipitation with poh/anions. J Lipid Res 1970; 11:583-98.
  18. Kono Y. Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase. Arch Biochem Biophys 1978; 189-95.
  19. Montilla P, Barcos M, Munoz M, Castaneda I, Tunez. Red wine prevents brain oxidative stress and nephropathy in streptozotocin induced diabetic rats. J Biochem Mol Biol 2005; 539-44.
  20. Makni M, Sefi M, Fetoui H, Garoui ME, Gargouri KN. Flax and pumpkin seeds mixture ameliorates diabetic nephropathy in rats. Food Chem Toxicol 2010; 2407-12.
  21. Kumar BD, Mitra A, Manjunatha M. A comparative study of alpha-amylase inhibitory activities of common antidiabetic plants of Kharagpur 1 block. Int J Green Pharm 2010; 4:115–21.
  22. Tattersall R. Targets of therapy for NIDDM. Diabetes Res Cli Pract 1995; 8: 549-55.
  23. Randle PJ, Garland PB, Hales CN, Newsholme EA. The glucose fatty-acid cycle. Its role in insulin sensitivity and the metabolic disturbances of diabetes mellitus. Lancet 1963; 1:785-9.