A Scientific Insight into Step by Step Procedures for the Prediction of Cytochrome Enzyme Oriented Herb-Drug Interactions

Dhivya Dhanasekaran, Nila Ganamurali, Sarvesh Sabarathinam*

SRM College of Pharmacy, SRM IST, Kattankulathur, kancheepuram, Tamil Nadu, India.

Abstract: Background: Herbs are widely used by majority of the patient for their chronic illness at least once in their life time. Liver enzymes play a major role in the metabolism of xenobiotics. In vivo & In vitro technique prediction of drug interaction is widely practiced. Method: Since there is no standard database for the prediction of herb-drug interaction. Abroad literature survey is carried out to ensure the process of prediction of herb-drug interaction from the various databases. For the prediction of herb-drug interaction step by step, strategy is enumerated in this review. Discussion: The drug interaction potential of targeted drug estimated by in silico, in vitro, in vivo animal model and phase one trial. CYP450 enzyme inhibition potential was assessed using High throughput screening assay followed by drug binding affinities measured through in silico studies. Conclusion: This study gives the step-by-step approach to analyzing the drug interaction potential herbal medicine with CYP450 enzymes.

Keywords:CYP450, Healthy volunteers, Herb-drug interaction, In vivo, In vitro, In silico, Probe drug.


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