Triticum aestivum (wheat grass) Exhibited Anticancer Activity on Oral Cancer (KB) Cell Line

Sanjeevini A Hattarki1,*, Chetna Bogar2, Kishore Bhat2

1Maratha Mandal's Dental college and Research centre, Belgaum, Karnataka, India.
2CRL Lab Maratha Mandal's Dental college and Research centre, Karnataka, India

Abstract:Objective: Currently oral cancer is the sixth most common cancer in the world with a quarter of world burden being borne by India. Wheat grass, a traditional medicine is used worldwide to treat various ailments, with its anticancer activity being reported on few cell lines like Hep2, Hela and K562. Aim: Aim of this study was to evaluate the anticancer and cytotoxic activity of WG on oral cancer (KB) cells and mouse embryonic cells (NIH3T3) respectively. Methods and Material: Standard KB and NIH3T3 cells lines were procured. Ethanol extract of freshly grown WG was prepared in our laboratory and commercially available powder of WG was also procured. Anticancer activity on KB cells and cytotoxic activity on NIH3T3 cells was evaluated by MTT assay with the two forms of WG. Statistical analysis used: Statistical analysis was performed by using spearman’s coefficient correlation to correlate the concentration of extract and cell inhibition. Results: WG exhibited an inhibition of KB cells in a dose-dependent manner with an IC50 value of 156µg/ml and was non-toxic on NIH3T3 cells. Conclusion: WG showed anticancer activity on KB cells and therefore can be considered for further studies on animals and eventually on human beings.

Keywords: Cell death, KB cells, NIH3T3 cells, Squamous cell carcinoma, Triticum aestivum.


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